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Title 

Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions

 

덱스트란나아제 및 환상올리고당 합성반응을 촉매하는 Bacteroides thetaiotaomicron VPI-5482 유래 효소

Authors 

Young Min KimE YamamotoM S KangH NakaiW SaburiM OkuyamaH MoriK FunaneM MommaZ FujimotoM KobayashiD KimA Kimura

Publisher 

Wiley-Blackwell

Issue Date 

2012

Citation 

FEBS Journal, vol. 279, no. 0, pp. 3185-3191

Keywords 

Bacteroides thetaiotaomicron VPI-5482cycloisomaltooligosaccharide glucanotransferaseendo-dextranaseglycoside hydrolase family 66isomaltotetraose

Abstract 

Bacteroides thetaiotaomicron VPI-5482 harbors a gene encoding a putative cycloisomaltooligosaccharide glucanotransferase (BT3087) belonging to glycoside hydrolase family 66. The goal of the present study was to characterize the catalytic properties of this enzyme. Therefore, we expressed BT3087 (recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482) in Escherichia coli and determined that recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482 preferentially synthesized isomaltotetraose and isomaltooligosaccharides (degree of polymerization > 4) from dextran. The enzyme also generated large cyclic isomaltooligosaccharides early in the reaction. We conclude that members of the glycoside hydrolase 66 family may be classified into three types: (a) endo-dextranases, (b) dextranases possessing weak cycloisomaltooligosaccharide glucanotransferase activity, and (c) cycloisomaltooligosaccharide glucanotransferases.

ISSN 

1742-464X

Link 

http://dx.doi.org/10.1111/j.1742-4658.2012.08698.x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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