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Title 

Humanization by guided selections

Authors 

Sang Jick KimH J Hong

Publisher 

Humana Press

Issue Date 

2012

Citation 

Methods in Molecular Biology, vol. 907, no. 0, pp. 247-257

Keywords 

Antibody libraryChain shufflingGuided selectionHuman antibodyPanningPhage display --------------------------------------------------------------------------------

Abstract 

Guided selection provides a powerful tool for humanization of the preexisting nonhuman antibodies as exemplified by HUMIRA, the world's first human antibody approved. This chapter describes the sequential guided selection procedure in which mouse VL and VH domains are replaced sequentially with human VL and VH, respectively to derive completely human antibody. The detailed protocols for construction of phage-displayed antibody library, panning, screening, and characterization, are included to achieve successful selection of human antibody with similar characteristics to original mouse antibody.

ISSN 

1064-3745

Link 

http://dx.doi.org/10.1007/978-1-61779-974-7-14

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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