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Title 

Dynamics of Setdb1 expression in early mouse development

Authors 

Sunwha ChoJung Sun ParkSujin KwonYong-Kook Kang

Publisher 

Elsevier

Issue Date 

2012

Citation 

Gene Expression Patterns, vol. 12, no. 5, pp. 213-218

Keywords 

Blastocyst outgrowthEmbryonic stem cellHistone methylationMouse embryoSetdb1/Eset --------------------------------------------------------------------------------

Abstract 

Setdb1/Eset, a histone lysine methyltransferase, is recruited by various transcription factors to modify local chromatin. The observation that Setdb1-null blastocysts fail to produce epiblast-lineage cells suggests a role for Setdb1 in generating mouse embryonic stem cells (mESCs). When examined in mouse zygotes, Setdb1 proteins appeared as dots at perinucleolar rims of pronuclei, with the dot-shaped signals more prominent in male pronuclei. Setdb1 signals were observed diffusely in the nucleus from the two-cell stage onward and, by the blastocyst, took a punctate form, away from nucleolus. Such varying expression patterns suggest its involvement in diverse biological processes at preimplantation stage. Setdb1 appeared in Oct4-positive cells of inner-cell-mass origin but not in trophectoderm-lineage cells in blastocyst outgrowths. Setdb1 co-immunoprecipitated with Oct4 in mESCs, and Setdb1 expressionwas markedly reduced upon retinoic acid-induced differentiation. These observations suggest that Setdb1 has an important role in maintaining the self-renewal of mESCs through collaboration with Oct4.

ISSN 

1567-133X

Link 

http://dx.doi.org/10.1016/j.gep.2012.03.005

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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