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Title 

An application of β-glycosidase to transformation of ginsenosides for the effective production of specific ginsenosides with biological efficacy

Authors 

Y HerY C LeeJ H OhY E ChoiChang Woo LeeJ S KimH M KimJ W Yang

Publisher 

Springer Verlag (Germany)

Issue Date 

2012

Citation 

Biotechnology and Bioprocess Engineering, vol. 17, no. 3, pp. 538-546

Keywords 

Enzymatic processGinsenoside combinationGinsenosidesLung cancer cell line (NCI-H23)PurificationSolvent system

Abstract 

Over the past several decades, the pharmacological effects of ginsenosides in Panax ginseng roots have been extensively investigated. Here, we developed a method for producing specific ginsenosides (F1 and F2) with good yields (F1:162 mg/g, F2:305 mg/g) using β-glycosidase purified from Aspergillus niger. In addition, each ginsenoside (at least 25 species) was separated and purified by high performance liquid chromatography (HPLC) using five different types of solvents and different purification steps. In addition, the Rg3:Rh2 mixture (1:1, w/w) was shown to inhibit a specific lung cancer cell line (NCI-H232) in vivo, displaying an anticancer effect at a dose lower than achieved using treatments with single Rg3 or Rh2. This finding suggests that the combination of ginsenosides for targeting anticancer is more effective than the use of a single ginsenoside from ginseng or red ginseng.

ISSN 

1226-8372

Link 

http://dx.doi.org/10.1007/s12257-011-0678-2

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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