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Title 

Virulence and transmissibility of H1N2 influenza virus in ferrets imply the continuing threat of triple-reassortant swine viruses

Authors 

P N Q PascuaaM S SongJ H LeeY H BaekH I KwonS J ParkE H ChoiG J LimO J LeeS W KimC J KimM H SungMyung Hee KimS W YoonE A GovorkovaR J WebbyR G WebsterY K Choi

Publisher 

National Academy of Sciences

Issue Date 

2012

Citation 

Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 39, pp. 15900-15905

Keywords 

EvolutionInterspecies transmissionSevere diseaseViral segments

Abstract 

Efficient worldwide swine surveillance for influenza A viruses is urgently needed; the emergence of a novel reassortant pandemic H1N1 (pH1N1) virus in 2009 demonstrated that swine can be the direct source of pandemic influenza and that the pandemic potential of viruses prevalent in swine populations must be monitored. We used the ferret model to assess the pathogenicity and transmissibility of predominant Korean triple-reassortant swine (TRSw) H1N2 and H3N2 influenza viruses genetically related to North American strains. Although most of the TRSw viruses were moderately pathogenic, one [A/Swine/Korea/1204/ 2009; Sw/1204 (H1N2)] was virulent in ferrets, causing death within 10 d of inoculation, and was efficiently transmitted to naive contact ferrets via respiratory droplets. Although molecular analysis did not reveal known virulence markers, the Sw/1204 virus acquired mutations in hemagglutinin (HA) (Asp-225-Gly) and neuraminidase (NA) (Ser-315-Asn) proteins during the single ferret passage. The contact-Sw/1204 virus became more virulent in mice, replicated efficiently in vitro, extensively infected human lung tissues ex vivo, and maintained its ability to replicate and transmit in swine. Reverse-genetics studies further indicated that the HA 225G and NA 315N substitutions contributed substantially in altering virulence and transmissibility. These findings support the continuing threat of some field TRSw viruses to human and animal health, reviving concerns on the capacity of pigs to create future pandemic viruses. Apart from warranting continued and enhanced global surveillance, this study also provides evidence on the emerging roles of HA 225G and NA 315N as potential virulence markers in mammals.

ISSN 

0027-8424

Link 

http://dx.doi.org/10.1073/pnas.1205576109

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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