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Title 

A nobel gebberellin 2-oxidase gene CaGA2ox1 in pepper is specifically induced by incompatible plant pathogens

 

고추 GA2-oxidase 유전자 연구

Authors 

Yeon LeeYoung-Cheol KimS Y KimI J LeeD ChoiK H PaekHye Sun ChoSuk Yoon KwonJeong Mee Park

Publisher 

Springer Verlag (Germany)

Issue Date 

2012

Citation 

Plant Biotechnology Reports, vol. 6, no. 6, pp. 381-385

Keywords 

CaGA2ox1Chili pepper (Capsicum annum)Gibberellin (GA)Non-host pathogen

Abstract 

Phytohormone balance is increasingly recognized as central to the outcome of plant-pathogen interactions. Differential screening for genes induced by a non-host pathogen in pepper plants (Capsicum annuum) identified a putative gibberellin 2-oxidase gene, CaGA2ox1. Analysis of the deduced amino acid sequence of CaGA2ox1 showed 53 and 50 % amino acid identity to Pisum sativum PsGA2ox2 and Arabidopsis AtGA2ox6, respectively. Expression in pepper plants of CaGA2ox1 was preferentially increased in response to non-host pathogeninoculation and during the host resistance response. CaGA2ox1 expression increased following treatment with salicylic acid and ethephon (albeit with different induction patterns), but remained unchanged following treatment with methyl jasmonate and abscisic acid. The gene product of CaGA2ox1 is predicted to catalyze the metabolism of GA 4, and does so in recombinant E. coli extracts. Further PEG-mediated transient expression studies showed that CaGA2ox1 fused with soluble modified green fluorescent protein localized to the cytosol in chili pepper protoplasts. Interestingly, the transcript level of CaGA2ox1 was not affected by treatments of either pepper with bioactive GA 4+7 or paclobutrazol, an inhibitor of GA biosynthesis. Taken together, these results provide the first evidence that a GA 2-oxidase, which is important in GA metabolism, may also play a role in plant defense signaling and plant-microbe interactions.

ISSN 

1863-5466

Link 

http://dx.doi.org/10.1007/s11816-012-0235-2

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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