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Title 

Inhibitory effects of epigallocatechin gallate and its glucoside on the human intestinal maltase inhibition

 

EGCG와 그 배당체의 인간 장내 말테이즈의 저해효과

Authors 

T T H NguyenS H JungS LeeH J RyuH K KangY H MoonYoung Min KimA KimurD Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2012

Citation 

Biotechnology and Bioprocess Engineering, vol. 17, no. 5, pp. 966-971

Keywords 

Alpha-glucosidaseEGCGEGCG glucosideHuman intestinal maltaseMolecular docking

Abstract 

Human intestinal maltase (HMA) is an α- glucosidase responsible for the hydrolysis of α-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA has become an important target in the treatment of type-2 diabetes. In this study, epigallocatechin gallate (EGCG) and EGCG glucoside (EGCG-G1) were identified as inhibitors of HMA by an in vitro assay with IC50 of 20 ± 1.0 and 31.5 ± 1.0 μM, respectively. A Lineweaver-Burk plot confirmed that EGCG and EGCG-G1 were competitive inhibitors of maltose substrate against HMA and inhibition kinetic constants (Ki) calculated from a Dixon plot were 5.93 ± 0.26 and 7.88 ± 0.57 μM, respectively. Both EGCG and EGCG-G1 bound to the active site of HMA with numerous hydrophobic and hydrogen bond interactions.

ISSN 

1226-8372

Link 

http://dx.doi.org/10.1007/s12257-012-0242-8

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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