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Title 

Stable expression of a fungal laccase protein using transplastomic tobacco

Authors 

S J DavarpanaJoon Woo AhnS M KoS H JungY I ParkJang Ryol LiuWon Joong Jeong

Publisher 

Springer Verlag (Germany)

Issue Date 

2012

Citation 

Plant Biotechnology Reports, vol. 6, no. 4, pp. 305-312

Keywords 

Heterologous expressionHomoplasmyLaccaseTransplastomic plant

Abstract 

Laccase catalyzes the oxidation of various phenolic compounds that can be used in a wide range of industrial applications such as waste detoxification and the textile industry. In the present study, we generated transplastomic tobacco plants to develop a reliable commercial source of laccase production. The stability of the laccase protein in the transgenic plants was increased by using the enhancer sequence from green fluorescent protein, resulting in three independent lines with high levels of laccase accumulation (up to 2 % of total protein); significant laccase activity, however, was not detected. Interestingly, the transplastomic lines showed slightly retarded vegetative growth, with a light green leaf color in comparison with the control, which may be attributable to copper deficiency induced by ligand chelation by abundantly produced laccase. These results suggest that the tobacco chloroplast is an efficient system for the mass production of laccase protein, but further studies are needed to obtain active enzyme.

ISSN 

1863-5466

Link 

http://dx.doi.org/10.1007/s11816-012-0225-4

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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