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Title 

Transient erythropoietin overexpression with cucumber mosaic virus suppressor 2b in Nicotiana benthamiana

 

오이 모자이크 바이러스 2b 억제에 의한 EPO의 임시발현

Authors 

Ki Beom MoonJae Heung JeonW S LeeHyun Soon Kim

Publisher 

Korean Society for Horticultural Science

Issue Date 

2012

Citation 

Horticulture, Environment, and Biotechnology, vol. 53, no. 5, pp. 434-440

Keywords 

Agrobacteriumbiopharmaceuticalsposttranscriptional gene silencingrecombinant proteins

Abstract 

Erythropoietin (EPO) is a glycoprotein hormone that regulates red blood cell formation in mammals. It also plays an important role in wound healing and the brain's response to neuronal injury. Recombinant EPO has been produced in various hosts including Escherichia coli, insect cells, and yeast. Plant systems are also useful for the production of recombinant proteins. In this study, we used Nicotiana benthamiana for the production of recombinant human EPO (rhEPO) using an Agrobacterium-mediated transient expression system. We evaluated the effect of repeated agroinfiltration and Agrobacterium density on the rhEPO production. In addition, the rhEPO expression vector was coinfiltrated with a vector expressing cucumber mosaic virus suppressor 2b (CMV2b), which suppresses posttranscriptional gene silencing. We found that rhEPO expression was increased approximately 5. 5-fold in N. benthamiana leaves coinfiltrated with CMV2b. In contrast, neither Agrobacterium density nor the number of infiltrations influenced rhEPO expression.

Citation 

ISSN 

0253-6498

Link 

http://dx.doi.org/10.1007/s13580-012-0971-9

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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