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Title 

NDRG2 and PRA1 interact and synergistically inhibit T-cell factor/b-catenin signaling

Authors 

Jong Tae KimJae Wha KimYun Hee KangK D KimSeon-Jin LeeS C ChoiK S KimS K ChaeJ W KimJ S LimHee Gu Lee

Publisher 

Elsevier

Issue Date 

2012

Citation 

FEBS Letters, vol. 586, no. 0, pp. 3962-3968

Keywords 

Cell proliferationNDRGPRA1Promoter inhibitionProtein-protein interactionYeast two-hybrid screen

Abstract 

NDRG2 is a member of the N-myc downstream regulated gene (NDRG) family, implicated in cell growth and differentiation. Investigation of NDRG2 molecular interactions by yeast two-hybrid screening identified prenylated Rab acceptor-1 (PRA1), involved in vesicle trafficking and protein transport, as binding partner. Binding of NDRG2 (and NDRG1-4) with PRA1 in vitro was confirmed by GST pull-down assay and immunoprecipitation, and colocalization was verified by confocal microscopy in HCT116 cells. Intracellular coexpression showed that NDRG2 and PRA1 synergistically downregulate T-cell factor (TCF) promoter activity and GSK3β phosphorylation. Results suggest that NDRG2 and PRA1 might act synergistically to prevent signaling of TCF/β-catenin. Structured summary of protein interactions: NDRG2a binds to PRA1 by pull down (View interaction) NDRG2a physically interacts with PRA1 by two hybrid (View Interaction: 1, 2) PRA1 physically interacts with NDRG2a by anti tag coimmunoprecipitation (View interaction) NDRG2a, PRA1 and Catenin beta colocalize by cosedimentation (View interaction) NDRG2a and PRA1 colocalize by fluorescence microscopy (View Interaction: 1, 2, 3)

ISSN 

0014-5793

Link 

http://dx.doi.org/10.1016/j.febslet.2012.09.045

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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