상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene

Authors 

M ChaS H ShimS H KimO T KimS W LeeSuk Yoon KwonK H Baek

Publisher 

Korean Society for Biochemistry and Molecular Biology

Issue Date 

2012

Citation 

BMB Reports, vol. 45, no. 10, pp. 589-594

Keywords 

Panax ginseng C.A. MeyerRoot cultureTaxadiene synthaseTaxolTransformation --------------------------------------------------------------------------------

Abstract 

Paclitaxel is produced by various species of yew trees and has been extensively used to treat tumors. In our research, a taxadiene synthase (TS) gene from Taxus brevifolia was used to transform the roots of cultured ginseng (Panax ginseng C.A. Meyer) to produce taxadiene, the unique skeletal precursor to taxol. The TS gene was successfully introduced into the ginseng genome, and the de novo formation of taxadiene was identified by mass spectroscopy profiling. Without any change in phenotypes or growth difference in a TS-transgenic ginseng line, the transgenic TSS3-2 line accumulated 9.1 μg taxadiene per gram of dry weight. In response to the treatment of methyl jasmonate for 3 or 6 days, the accumulation was 14.6 and 15.9 μg per g of dry weight, respectively. This is the first report of the production of taxadiene by engineering ginseng roots with a taxadiene synthase gene.

ISSN 

1976-6696

Link 

http://dx.doi.org/10.5483/BMBRep.2012.45.10.085

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)