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Title 

Quantitative analyses of individual sugars in mixture using FRET-based biosensors

 

FRET 기반 바이오센서 이용 복합당 정량분석 기술

Authors 

J S HaJongsik GamSu-Lim ChoiK H OhH S RoJae Jun SongC S ShinSeung Goo Lee

Publisher 

American Chemical Society

Issue Date 

2012

Citation 

Biotechnology Progress, vol. 28, no. 5, pp. 1376-1383

Keywords 

BiomassFluorescence proteinFluorescence resonance energy transferMolecular biosensorSugar

Abstract 

Molecular biosensors were developed and applied to measure individual sugars in biological mixtures such as bacterial culture broths. As the sensing units, four sugar-binding proteins (SBPs for allose, arabinose, ribose, and glucose) were selected from the Escherichia coli genome and connected to a cyan fluorescent protein and yellow fluorescent protein via dipeptide linkers (CFP-L-SBP-YFP). The putative sensors were randomized in the linker region (L) and then investigated with regard to the intensity of fluorescence resonance energy transfer on the binding of the respective sugars. As a result, four representatives were selected from each library and examined for their specificity using 16 available sugars. The apparent dissociation constants of the allose, arabinose, ribose, and glucose sensors were estimated to be 0.35, 0.36, 0.17, and 0.18 μM. Finally, the sugar sensors were applied to monitor the consumption rate of individual sugars in an E. coli culture broth. The individual sugar profiles exhibited a good correlation with those obtained using an HPLC method, confirming that the biosensors offer a rapid and easy-to-use method for monitoring individual sugars in mixed compositions.

ISSN 

8756-7938

Link 

http://dx.doi.org/10.1002/btpr.1592

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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