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Title 

Screening of tissue-specific genes and promoters in tomato by comparing genome wide expression profiles of arabidopsis orthologues

Authors 

Chan Ju LimHa Yeon LeeWoong Bom KimB S LeeJungeun KimR AhmadHyun A KimSo Young YiCheol-Goo HurSuk Yoon Kwon

Publisher 

Springer Verlag (Germany)

Issue Date 

2012

Citation 

Molecules and Cells, vol. 34, no. 1, pp. 53-59

Keywords 

orthologue searchpromotertissue-specific genetomato

Abstract 

Constitutive overexpression of transgenes occasionally interferes with normal growth and developmental processes in plants. Thus, the development of tissue-specific promoters that drive transgene expression has become agriculturally important. To identify tomato tissue-specific promoters, tissue-specific genes were screened using a series of in silico-based and experimental procedures, including genome-wide orthologue searches of tomato and Arabidopsis databases, isolation of tissue-specific candidates using an Arabidopsis microarray database, and validation of tissue specificity by reverse transcription-polymerase chain reaction (RT-PCR) analysis and promoter assay. Using these procedures, we found 311 tissue-specific candidate genes and validated 10 tissuespecific genes by RT-PCR. Among these identified genes, histochemical analysis of five isolated promoter::GUS transgenic tomato and Arabidopsis plants revealed that their promoters have different but distinct tissue-specific activities in anther, fruit, and root, respectively. Therefore, it appears these in silico-based screening approaches in addition to the identification of new tissue-specific genes and promoters will be helpful for the further development of tailored crop development.

ISSN 

1016-8478

Link 

http://dx.doi.org/10.1007/s10059-012-0068-4

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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