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Title 

Homologous alkalophilic and acidophilic L-arabinose isomerases reveal region-specific contributions to the pH dependence of activity and stability

Authors 

S J LeeSang Jun LeeYong-Jik LeeS B KimS K KimD W Lee

Publisher 

American Society for Microbiology

Issue Date 

2012

Citation 

Applied and Environmental Microbiology, vol. 78, no. 24, pp. 8813-8816

Abstract 

To study the pH dependence of L-arabinose isomerase (AI) activity and stability, we compared homologous AIs with their chimeras. This study demonstrated that an ionizable amino acid near the catalytic site determines the optimal pH (pHopt) for activity, whereas the N-terminal surface R residues play an important role in determining the pHopt for stability.

ISSN 

0099-2240

Link 

http://dx.doi.org/10.1128/AEM.02114-12

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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