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Title 

Enhancement of geraniol resistance of Escherichia coli by MarA overexpression

Authors 

A A ShahC WangY R ChungJ Y KimEui Sung ChoiS W Kim

Publisher 

Elsevier

Issue Date 

2013

Citation 

Journal of Bioscience and Bioengineering, vol. 115, no. 3, pp. 253-258

Keywords 

Efflux pumpEscherichia coliGeraniolMarATerpenoid tolerance

Abstract 

Improvement of a microorganism's tolerance against organic solvents is required for a microbial factory producing terpenoid based biofuels. The bacterial genes, marA, imp, cls and cti have been found to increase organic solvent tolerance. Thus, the tolerance against the following terpenoids (isopentenol, geraniol, myrcene, and farnesol) was studied with overexpression of marA, imp, cls and cti genes in Escherichia coli. The marA overexpression significantly enhanced the tolerance of E. coli against geraniol, whereas there was no tolerance improvement against the terpenoids by overexpression of cls and cti genes. The imp overexpression even yielded sensitive phenotype to the tested solvents. The colony forming efficiency of the marA overexpressing E. coli was increased by 104-fold in plate overlay of geraniol compared to that of wild type E. coli and a two-fold decrease of intracellular geraniol accumulation was also observed in liquid culture of geraniol. Single knock-out mutations of marA, or one of the following genes (acrA, acrB and tolC) encoding AcrAB-TolC efflux pump made E. coli hypersensitive to geraniol. The geraniol tolerance conferred by marA overexpression was attributed to the AcrAB-TolC efflux pump that is activated by MarA.

ISSN 

1389-1723

Link 

http://dx.doi.org/10.1016/j.jbiosc.2012.10.009

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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