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Title 

GAL promoter-driven heterologous gene expression in Saccharomyces cerevisiae Δ strain at anaerobic alcoholic fermentation

Authors 

Jungoh AhnKyung Min ParkHong-Weon LeeY J SonEui Sung Choi

Issue Date 

2013

Citation 

FEMS YEAST RESEARCH, vol. 13, no. 1, pp. 140-142

Keywords 

Δgal80Anaerobic alcoholic fermentationGAL10 promoterSaccharomyces cerevisiae --------------------------------------------------------------------------------

Abstract 

The removal of Gal80 protein by gene disruption turned into efficient GAL promoter-driven heterologous gene expression under anaerobic alcoholic fermentation of Saccharomyces cerevisiae. Using lipase B from Candida antarctica as a reporter, the relative strength of GAL10 promoter (PGAL10) in Δgal80 mutant that does not require galactose as an inducer was compared to those of ADH1, PDC1, and PGK promoters, which have been known to work well anaerobically in actively fermenting yeast cells under high glucose concentration. PGAL10 in the Δgal80 mutant showed 0.8-fold (ADH1), fourfold (PDC1), and 50-fold (PGK) in promoter strength.

ISSN 

1567-1356

Link 

http://dx.doi.org/10.1111/1567-1364.12009

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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