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Title 

pH-dependent structural change of the extracellular sensor domain of the DraK histidine kinase from Streptomyces coelicolor

 

스트렙토마이세스 실리칼라의 DraK 히스티딘 카이나아제의 세포외 센서도메인의 pH 의존성 구조변화

Authors 

K J YeoEun Hye KimE HwangY H HanY EoH J KimOh Suk KwonYoung-Soo HongC CheongH K Cheong

Publisher 

Elsevier

Issue Date 

2013

Citation 

Biochemical and Biophysical Research Communications, vol. 431, no. 3, pp. 554-559

Keywords 

DraKPH-dependent conformational changePH-sensitive histidine kinaseSCO3062Sensory domainStreptomyces coelicolorTwo-component system

Abstract 

Recently, the DraR/DraK (Sco3063/Sco3062) two-component system (TCS) of Streptomyces coelicolor has been reported to be involved in the differential regulation of antibiotic biosynthesis. However, it has not been shown that under which conditions and how the DraR/DraK TCS is activated to initiate the signal transduction process. Therefore, to understand the sensing mechanism, structural study of the sensory domain of DraK is highly required. Here, we report the biochemical and biophysical properties of the extracellular sensory domain (ESD) of DraK. We observed a reversible pH-dependent conformational change of the ESD in a pH range of 2.5-10. Size-exclusion chromatography and AUC (analytical ultracentrifugation) data indicated that the ESD is predominantly monomeric in solution and exists in equilibrium between monomer and dimer states in acidic condition. Using NMR (nuclear magnetic resonance) and CD (circular dichroism) spectroscopy, our findings suggest that the structure of the ESD at low pH is more structured than that at high pH. In particular, the glutamate at position 83 is an important residue for the pH-dependent conformational change. These results suggest that this pH-dependent conformational change of ESD may be involved in signal transduction process of DraR/DraK TCS.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2013.01.018

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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