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Title 

Novel cell penetrating peptides with multiple motifs composed of RGD and its analogs

Authors 

A A MokhtariehSemi KimYunhee LeeBong Hyun ChungMyung Kyu Lee

Publisher 

Elsevier

Issue Date 

2013

Citation 

Biochemical and Biophysical Research Communications, vol. 432, no. 2, pp. 359-364

Keywords 

Cell penetrating peptide (CPP)CPP-mediated liposome uptakeGene silencingPeptide cytotoxicityRGD motif

Abstract 

Cell penetrating peptides (CPPs) have been used to transport macromolecules into cells. Most CPPs have properties such as a strong polycationic charge, amphipathic basic, and hydrophobicity. In this study, we designed the peptides with multiple motifs composed of RGD and its analogs to induce integrin-mediated endocytosis as well as endosomal escape by forming an amphipathic helix in acidic endosomes. These peptides were proved less toxic to animal cells than those without acidic residues. Unexpectedly, peptide conjugated liposomes could penetrate into cells regardless of integrins. The replacement of all aspartic acids by glutamic acids did not prevent the peptide-mediated liposome uptake, and the higher basic and leucine contents enhanced the gene silencing activity of siRNA encapsulated in the liposomes. The peptide is considered to be a new type of CPP which can be used for drug delivery.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2013.01.096

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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