상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Efficient production of 1,3-propanediol from glycerol upon constitutive expression of the 1,3-propanediol oxidoreductase gene in engineered Klebsiella pneumoniae with elimination of by-product formation

Authors 

Baek Rock OhJeong-Woo SeoSun-Yeon HeoL H LuoWon-Kyung HongD H ParkChul Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2013

Citation 

Bioprocess and Biosystems Engineering, vol. 36, no. 6, pp. 757-763

Keywords 

1,3-PropanediolBy-product formationConstitutive expressionGlycerolKlebsiella pneumoniae

Abstract 

In the present study, we developed an efficient method of 1,3-propanediol (1,3-PD) production from glycerol by genetic engineering of Klebsiella pneumoniae AK mutant strains. The proposed approach eliminated by-product formation and IPTG induction resulted in maximal production of 1,3-PD. A series of recombinant strains was designed to constitutively express the dhaB and/or dhaT genes, using the bacteriophage T5 PDE20 promoter and the rho-independent transcription termination signal of the Rahnella aquatilis levansucrase gene. Among these strains, AK/pConT expressing dhaT alone gave the highest yield of 1,3-PD. Fed-batch fermentation resulted in efficient production of 1,3-PD from either pure or crude glycerol, without by-product formation.

ISSN 

1615-7591

Link 

http://dx.doi.org/10.1007/s00449-013-0901-y

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)