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Title 

Computational prediction for the protein interactions of tyrosinase: protein experimental interactome MAP

Authors 

W WangD ParkSunyoung JiS J YinG Y QianH Y ChungJ M YangJinhyuk LeeY D Park

Publisher 

Elsevier

Issue Date 

2013

Citation 

Process Biochemistry, vol. 48, no. 4, pp. 638-648

Keywords 

InteractomicsPEIMAPProtein-protein interactionSimulationTyrosinase

Abstract 

Tyrosinase (EC 1.14.18.1) is a diversely distributed enzyme in various organisms with physiological roles related to pigment production. Tyrosinase has gained the attention of researchers due to its biological functions and potential applications. In this regard, studies on the partner proteins of tyrosinase are important. In this study, we predicted the 3D structure of human tyrosinase and simulated the protein-protein interactions between tyrosinase and binding partners by using the PEIMAP algorithm. As a result, we found that tyrosinase is predicted to bind with G protein-related proteins, potassium voltage-gated channel-related proteins, and vesicle/sorting-related proteins. In particular, GIPC1, GIPC2, GIPC3, TYRP1, and DCT were predicted to primarily bind with tyrosinase. Interacting proteins (103) were secondarily bound to these 5 interacting proteins in the PEIMAP network of tyrosinase. An involvement in melanogenesis was also newly predicted by associating the predicted binding proteins. We simulated the protein-protein bindings by probing the residues of each complex facing toward the predicted site of interaction with tyrosinase. Among the interacting residues, some were predicted to dock to the active site of tyrosinase, which could affect its activity directly. Our computational predictions will be useful for elucidating the protein-protein interactions of tyrosinase and for studying its binding mechanisms and the melanin biosynthesis pathway.

ISSN 

0032-9592

Link 

http://dx.doi.org/10.1016/j.procbio.2013.02.030

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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