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Title 

Characterization of a squalene synthase from the thraustochytrid microalga Aurantiochytrium sp. KRS101

Authors 

Won-Kyung HongSun-Yeon HeoHye Mi ParkChul Ho KimJung Hoon SohnA KondoJeong-Woo Seo

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2013

Citation 

Journal of Microbiology and Biotechnology, vol. 23, no. 6, pp. 759-765

Keywords 

AurantiochytriumFunctional characterizationGene analysisSqualene synthase

Abstract 

The gene encoding squalene synthase (SQS) of the lipidproducing heterotrophic microalga Aurantiochytrium sp. KRS101 was cloned and characterized. The krsSQS gene is 1,551 bp in length and has two exons and one intron. The open reading frame of the gene is 1,164 bp in length, yielding a polypeptide of 387 predicted amino acid residues with a molecular mass of 42.7 kDa. The deduced krsSQS sequence shares at least four conserved regions known to be required for SQS enzymatic activity in other species. The protein, tagged with His6, was expressed into soluble form in Escherichia coli. The purified protein catalyzed the conversion of farnesyl diphosphate to squalene in the presence of NADPH and Mg2+. This is the first report on the characterization of an SQS from a Thraustochytrid microalga

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1212.12023

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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