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Title 

Novel approach for the development of axenic microalgal cultures from environmental samples

Authors 

Dae Hyun ChoR RamananByung-Hyuk KimJimin LeeSora KimChan YooG G ChoiHee-Mock OhHee-Sik Kim

Publisher 

Wiley-Blackwell

Issue Date 

2013

Citation 

Journal of Phycology, vol. 49, no. 4, pp. 802-810

Keywords 

Axenic cultureChlorella sp.FACSMicroalgaeMicropickingScenedesmus sp.Ultrasonication

Abstract 

We demonstrated a comprehensive approach for development of axenic cultures of microalgae from environmental samples. A combination of ultrasonication, fluorescence-activated cell sorting (FACS), and micropicking was used to isolate axenic cultures of Chlorella vulgaris Beyerinck (Beijerinck) and Chlorella sorokiniana Shihira & R.W. Krauss from swine wastewater, and Scenedesmus sp. YC001 from an open pond. Ultrasonication dispersed microorganisms attached to microalgae and reduced the bacterial population by 70%, and when followed by cell sorting yielded 99.5% pure microalgal strains. The strains were rendered axenic by the novel method of micropicking and were tested for purity in both solid and liquid media under different trophic states. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene confirmed the absence of unculturable bacteria, whereas fluorescence microscopy and scanning electron microscopy (SEM) further confirmed the axenicity. This is the most comprehensive approach developed to date for obtaining axenic microalgal strains without the use of antibiotics and repetitive subculturing.

ISSN 

0022-3646

Link 

http://dx.doi.org/10.1111/jpy.12091

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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