상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Enhanced kinase sensing by radio-phosphorylation and oriented immobilization of a GBP-linked kinase substrate on a gold surface

Authors 

J K RhoJ A KangM H ChoiKyong-Cheol KoS H Park

Publisher 

Elsevier

Issue Date 

2011

Citation 

Sensors and Actuators B-Chemical, vol. 160, no. 0, pp. 632-636

Keywords 

BiochipGold-binding peptideOriented immobilizationPhosphorylationRadioisotope detection

Abstract 

A new strategy for enhanced sensing of kinases was developed using radio-phosphorylation and a polypeptide with an affinity for gold that was linked directly to kinase substrates. Gold-binding polypeptide (GBP)-linked GST-fusion substrates were efficiently immobilized on gold-coated slides, which is highly desirable for protein chips. The novel fusion substrates permitted oriented substrate immobilization on gold-coated slides, thereby enhancing the sensitivity of kinase assays. Thus, the proposed strategy is applicable to the development of protein microarrays and microfluidic lab-on-a-chip (LOC) sensing the activities of protein kinases.

ISSN 

0925-4005

Link 

http://dx.doi.org/10.1016/j.snb.2011.08.040

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)