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Title 

Proteomic analysis of acetylation in thermophilic Geobacillus kaustophilus

Authors 

Dong-Woo LeeD KimY J LeeJung-Ae KimJi Young ChoiSunghyun KangJae Gu Pan

Publisher 

Wiley-Blackwell

Issue Date 

2013

Citation 

Proteomics, vol. 13, no. 15, pp. 2278-2282

Keywords 

AcetylationGeobacillus kaustophilusMicrobiologyPosttranslational modificationProteomeThermophile --------------------------------------------------------------------------------

Abstract 

Recent analysis of prokaryotic Nε-lysine-acetylated proteins highlights the posttranslational regulation of a broad spectrum of cellular proteins. However, the exact role of acetylation remains unclear due to a lack of acetylated proteome data in prokaryotes. Here, we present the Nε-lysine-acetylated proteome of gram-positive thermophilic Geobacillus kaustophilus. Affinity enrichment using acetyl-lysine-specific antibodies followed by LC-MS/MS analysis revealed 253 acetylated peptides representing 114 proteins. These acetylated proteins include not only common orthologs from mesophilic Bacillus counterparts, but also unique G. kaustophilus proteins, indicating that lysine acetylation is pronounced in thermophilic bacteria. These data complement current knowledge of the bacterial acetylproteome and provide an expanded platform for better understanding of the function of acetylation in cellular metabolism.

ISSN 

1615-9853

Link 

http://dx.doi.org/10.1002/pmic.201200072

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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