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Title 

Identification and characterization of Klebsiella pneumoniae aldehyde dehydrogenases increasing production of 3-hydroxypropionic acid from glycerol

Authors 

L L LuoJeong-Woo SeoSun-Yeon HeoB R OhD H KimChul Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2013

Citation 

Bioprocess and Biosystems Engineering, vol. 36, no. 9, pp. 1319-1326

Keywords 

3-Hydroxypropionic acidAldehyde dehydrogenaseGlycerolKlebsiella pneumoniae

Abstract 

Klebsiella pneumoniae produces 3-hydroxypropionic acid (3-HP) from glycerol with oxidation of 3-hydroxypropionaldehyde (3-HPA) to 3-HP in a reaction catalyzed by aldehyde dehydrogenase (ALDH). In the present study, two putative ALDHs of K. pneumoniae, YneI and YdcW were identified and characterized. Recombinant YneI was specifically active on 3-HPA and preferred NAD+ as a cofactor, whereas YdcW exhibited broad substrate specificity and preferred NADP+ as a cofactor. Overexpression of ALDHs in the glycerol oxidative pathway-deficient mutant K. pneumoniae AK resulted in a significant increase in 3-HP production upon shake-flask culture. The final titers of 3-HP were 2.4 and 1.8 g L-1 by recombinants overexpressing YneI and YdcW, respectively. Deletion of the ALDH gene from K. pneumoniae did not affect the extent of 3-HP synthesis, implying non-specific activity of ALDHs on 3-HPA. The ALDHs might play major roles in detoxifying the aldehyde generated in glycerol metabolism.

ISSN 

1615-7591

Link 

http://dx.doi.org/10.1007/s00449-012-0880-4

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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