상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative xylose isomerase from Bacteroides thetaiotaomicron

Authors 

J W ChoB G HanS Y ParkSeung Jun KimM D KimB I Lee

Publisher 

International Union of Crystallography

Issue Date 

2013

Citation 

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, vol. 69, no. 10, pp. 1127-1130

Keywords 

Bacteroides thetaiotaomicronBT0793glucose isomerasexylose isomerase

Abstract 

Bacteroides thetaiotaomicron BT0793, a putative xylose isomerase, was overexpressed in Escherichia coli, purified and crystallized using polyethylene glycol monomethyl ether 550 as the precipitant. X-ray diffraction data were collected to 2.10 ? resolution at 100 K using synchrotron X-rays. The crystal was found to belong to space group P1, with unit-cell parameters a = 96.3, b = 101.7, c = 108.3 ?, α = 82.8, β = 68.2, γ = 83.0°. The asymmetric unit contained eight subunits of xylose isomerase with a crystal volume per protein weight (V M) of 2.38 ?3 Da -1 and a solvent content of 48.3%.

Citation 

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 69(10): 1127-1130

ISSN 

1744-3091

Link 

http://dx.doi.org/10.1107/S1744309113023877

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


Files in This Item: SizeFormat
11906.pdf398KbAdobe PDF
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)