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Title 

Presence of transcription factor OCT4 limits interferon-tau expression during the pre-attachment period in sheep

Authors 

KimT SakuraiH BaiR BaiD SatoK NagaokaKyu Tae ChangJ D GodkinK S MinK Imakawa

Publisher 

The Asian-australasian Association of Animal

Issue Date 

2013

Citation 

Asian-Australasian Journal of Animal Sciences, vol. 26, no. 5, pp. 638-645

Keywords 

Gene regulationImplantationInterferon-tauSheepTranscription

Abstract 

Interferon-tau (IFNT) is thought to be the conceptus protein that signals maternal recognition of pregnancy in ruminants. We and others have observed that OCT4 expression persists in the trophectoderm of ruminants; thus, both CDX2 and OCT4 coexist during the early stages of conceptus development. The aim of this study was to examine the effect of CDX2 and OCT4 on IFNT gene transcription when evaluated with other transcription factors. Human choriocarcinoma JEG-3 cells were cotransfected with an ovine IFNT (-654-bp)-luciferase reporter (-654-IFNT-Luc) construct and several transcription factor expression plasmids. Cotransfection of the reporter construct with Cdx2, Ets2 and Jun increased transcription of -654-IFNT-Luc by about 12-fold compared with transfection of the construct alone. When cells were initially transfected with Oct4 (0 h) followed by transfection with Cdx2, Ets2 and/or Jun 24 h later, the expression of -654-IFNT-Luc was reduced to control levels. OCT4 also inhibited the stimulatory activity of CDX2 alone, but not when CDX2 was combined with JUN and/or ETS2. Thus, when combined with the other transcription factors, OCT4 exhibited little inhibitory activity towards CDX2. An inhibitor of the transcriptional coactivator CREB binding protein (CREBBP), 12S E1A, reduced CDX2/ETS2/JUN stimulated -654-IFNT-Luc expression by about 40%, indicating that the formation of an appropriate transcription factor complex is required for maximum expression. In conclusion, the presence of OCT4 may initially minimize IFNT expression; however, as elongation proceeds, the increasing expression of CDX2 and formation of the transcription complex leads to greatly increased IFNT expression, resulting in pregnancy establishment in ruminants.

ISSN 

1011-2367

Link 

http://dx.doi.org/10.5713/ajas.2012.12462

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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