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Title 

Patterns of protein expression in water-stressed wheat chloroplasts

Authors 

A H M KamalK ChoJ S ChoiY JinC S ParkJ S LeeS H Woo

Publisher 

Springer Verlag (Germany)

Issue Date 

2013

Citation 

Biologia Plantarum, vol. 57, no. 2, pp. 305-312

Keywords 

chloride carrier/channel familyH+-ATPaseMALDI-TOF/TOF-MSTriticum aestivum

Abstract 

The performance of control and water-stressed 10-d-old wheat seedlings was compared. During short-term water stress (irrigation was withheld for 9 d), rates of photosynthesis and transpiration, stomatal conductance, and relative water content decreased whereas the proline content increased. Chloroplast proteins were extracted from the leaves, separated by iso-electric focusing through two-dimensional electrophoresis, and stained with CBB R-250. Differentially expressed proteins were detected and analyzed with MALDI-TOF/TOF mass spectrometry. Under water stress, 9 proteins were up-regulated whereas 11 proteins were not affected. The ribulose-1,5-bisphospate carboxylase/oxygenase (Rubisco) small and large subunits, chloride carrier/channel family, and H+-ATPase were up-regulated by water stress whereas membrane-bound ATP synthase subunit b and cytochrome b6-f complex were down-regulated.

ISSN 

0006-3134

Link 

http://dx.doi.org/10.1007/s10535-012-0290-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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