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Title 

Methanobrevibacter boviskoreani sp. nov., isolated from the rumen of Korean native cattle

Authors 

Jong-Hwan LeeS KumarGeun Hye LeeDong-Ho ChangMoon Soo RheeM H YoonByoung Chan Kim

Publisher 

Society for General Microbiology

Issue Date 

2013

Citation 

International Journal of Systematic and Evolutionary Microbiology, vol. 63, no. 11, pp. 4196-4201

Abstract 

Three strictly anaerobic, methanogenic strains JH1T, JH4 and JH8 were isolated from rumen of the Korean native cattle (HanWoo; Bos taurus coreanae) in South Korea. The colonies were circular, opaque, and slightly yellowish. Phylogenetic analyses of 16S rRNA gene and mcrA (encoding α subunit of methyl-coenzyme M reductase) sequences confirmed the affiliation of the novel strains with the Methanobacteriales, and Methanobrevibacter wolinii SHT was the most closely related species. The 16S rRNA gene and mcrA sequence similarities between strains JH1T, JH4 and JH8 and M. wolinii SHT were 96.2 and 89.0% respectively, and DNA-DNA hybridization of the isolates and M. wolinii DSM 11976T showed a 20% reassociation. Strain JH1T exhibited 92% DNA-DNA relatedness with strains JH4 and JH8, and their 16S rRNA gene and mcrA sequences were identical. Cells stained Gram-positive and were non-motile rods, 1.5-1.8 mm long and 0.6 mm wide. The strains were able to use H2/CO2 and formate. The optimum temperature and pH ranges for growth were 37-40 °C and pH 6.5-7.0. The DNA G+C content of strain JH1T was 28 mol%. Based on data from this study using a polyphasic approach, the three strains represent a novel species of genus Methanobrevibacter, for which the name Methanobrevibacter boviskoreani sp. nov. is proposed. The type strain is JH1T (5KCTC 4102T5JCM 18376T)

ISSN 

1466-5026

Link 

http://dx.doi.org/10.1099/ijs.0.054056-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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