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Title 

Molecular cloning and functional characterization of an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935

Authors 

J B WooH N KangEui-Jeon WooS B Lee

Publisher 

Elsevier

Issue Date 

2014

Citation 

Food Chemistry, vol. 148, no. 0, pp. 184-187

Keywords 

β-1,3-GlucooligosaccharideAnti-fungalCandida albicansCurdlanEndo-β-1,3-glucanase

Abstract 

A gene encoding an endo-β-1,3-glucanase from Streptomyces matensis ATCC 23935 (SmβG) was characterised by cloning and expressing it in Escherichia coli. The purified enzyme produced β-1,3-glucooligosaccharides, mainly laminaripentaose, from insoluble curdlan. The optimum pH and temperature were 6.0 and 55 C, respectively. SmβG inhibited the growth of Candida albicans, which indicates that this enzyme could be potentially used as an anti-fungal agent to control invasive Candida infections. The results suggest that SmβG may be a useful bioavailable β-1,3-glucanase.

ISSN 

0308-8146

Link 

http://dx.doi.org/10.1016/j.foodchem.2013.09.137

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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