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Title 

Inactivation of metabolic genes causes short- and long- range dys-regulation in Escherichia coli metabolic network

 

대사유전자 비활성화로 인한 대사네트워크 유전자 조절에 미치는 영향

Authors 

D K BarupalSang Jun LeeE D KarolyS Adhya

Publisher 

Public Library of Science

Issue Date 

2013

Citation 

Plos One, vol. 8, no. 12, pp. e78360-e78360

Abstract 

The metabolic network in E. coli can be severely affected by the inactivation of metabolic genes that are required to catabolize a nutrient (D-galactose). We hypothesized that the resulting accumulation of small molecules can yield local as well as systemic effects on the metabolic network. Analysis of metabolomics data in wild-type and D-galactose non-utilizing mutants, galT, galU and galE, reveal the large metabolic differences between the wild-type and the mutants when the strains were grown in D-galactose. Network mapping suggested that the enzymatic defects affected the metabolic modules located both at short- and long-ranges from the D-galactose metabolic module. These modules suggested alterations in glutathione, energy, nucleotide and lipid metabolism and disturbed carbon to nitrogen ratio in mutant strains. The altered modules are required for normal cell growth for the wild-type strain, explaining why the cell growth is inhibited in the mutants in the presence of D-galactose. Identification of these distance-based dys-regulations would enhance the systems level understanding of metabolic networks of microorganisms having importance in biomedical and biotechnological research

ISSN 

1932-6203

Link 

http://dx.doi.org/10.1371/journal.pone.0078360

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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