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Title 

Protection of NAD(P)H: Quinone oxidoreductase 1 against renal ischemia/reperfusion injury in mice

Authors 

Gil Tae GangJung Hwan HwangYong-Hoon KimJung Ran NohKyoung Shim KimJ Y JeongD E ChoiK W LeeJ Y JungM ShongChul Ho Lee

Publisher 

Elsevier

Issue Date 

2014

Citation 

Free Radical Biology and Medicine, vol. 67, no. 1, pp. 139-149

Keywords 

β-LapachoneFree radicalsIschemia/reperfusion injuryNADPH oxidaseNQO1

Abstract 

Ischemia/reperfusion (I/R) is the most common cause of acute renal injury. I/R-induced reactive oxygen species (ROS) are thought to be a major factor in the development of acute renal injury by promoting the initial tubular damage. NAD(P)H:quinone oxidoreductase 1 (NQO1) is a well-known antioxidant protein that regulates ROS generation. The purpose of this study was to investigate whether NQO1 modulates the renal I/R injury (IRI) associated with NADPH oxidase (NOX)-derived ROS production in an animal model. We analyzed renal function, oxidative stress, and tubular apoptosis after IRI. NQO1-/- mice showed increased blood urea nitrogen and creatinine levels, tubular damage, oxidative stress, and apoptosis. In the kidneys of NQO1-/- mice, the cellular NADPH/NADP+ ratio was significantly higher and NOX activity was markedly higher than in those of NQO1+/+ mice. The activation of NQO1 by β-lapachone (βL) significantly improved renal dysfunction and reduced tubular cell damage, oxidative stress, and apoptosis by renal I/R. Moreover, the βL treatment significantly lowered the cellular NADPH/NADP+ ratio and dramatically reduced NOX activity in the kidneys after IRI. From these results, it was concluded that NQO1 has a protective role against renal injury induced by I/R and that this effect appears to be mediated by decreased NOX activity via cellular NADPH/NADP+ modulation. These results provide convincing evidence that NQO1 activation might be beneficial for ameliorating renal injury induced by I/R.

ISSN 

0891-5849

Link 

http://dx.doi.org/10.1016/j.freeradbiomed.2013.10.817

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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