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Title 

Engineering Escherichia coli for selective geraniol production with minimized endogenous dehydrogenation

Authors 

J ZhouC WangS H YoonH J JangEui Sung ChoiS W Kim

Publisher 

Elsevier

Issue Date 

2014

Citation 

Journal of Biotechnology, vol. 169, no. 1, pp. 42-50

Keywords 

E. coliGeraniolGeraniol dehydrogenationMevalonate pathwayMonoterpene

Abstract 

Geraniol, a monoterpene alcohol, has versatile applications in the fragrance industry, pharmacy and agrochemistry. Moreover, geraniol could be an ideal gasoline alternative. In this study, recombinant overexpression of geranyl diphosphate synthase and the bottom portion of a foreign mevalonate pathway in Escherichia coli MG1655 produced 13.3. mg/L of geraniol. Introduction of Ocimum basilicum geraniol synthase increased geraniol production to 105.2. mg/L. However, geraniol production encountered a loss from its endogenous dehydrogenization and isomerization into other geranoids (nerol, neral and geranial). Three E. coli enzymes (YjgB, YahK and YddN) were identified with high sequence identity to plant geraniol dehydrogenases. YjgB was demonstrated to be the major one responsible for geraniol dehydrogenization. Deletion of yjgB increased geraniol production to 129.7. mg/L. Introduction of the whole mevalonate pathway for enhanced building block synthesis from endogenously synthesized mevalonate improved geraniol production up to 182.5. mg/L in the yjgB mutant after 48. h of culture, which was a double of that obtained in the wild type control (96.5. mg/L). Our strategy for improving geraniol production in engineered E. coli should be generalizable for addressing similar problems during metabolic engineering.

ISSN 

0168-1656

Link 

http://dx.doi.org/10.1016/j.jbiotec.2013.11.009

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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