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Title 

Digital mRNA profiling of N-glycosylation gene expression in recombinant Chinese hamster ovary cells treated with sodium butyrate

Authors 

S M LeeYeon Gu KimEun Gyo LeeG M Lee

Publisher 

Elsevier

Issue Date 

2014

Citation 

Journal of Biotechnology, vol. 171, no. 1, pp. 56-60

Keywords 

CHO cellsGene expressionN-GlycosylationNanoString nCounter systemSodium butyrate

Abstract 

To understand the effects of sodium butyrate (NaBu) on protein glycosylation, recombinant Chinese hamster ovary (rCHO) cells producing Fc-fusion glycoprotein were subjected to 3. mM NaBu. The addition of NaBu to the cultures reduced the relative proportion of acidic isoforms and sialic acid content of the glycoprotein. Fifty-two N-glycosylation-related gene expressions were also assessed by the NanoString nCounter system, which can provide a direct digital readout using custom-designed color-coded probes. Among them, ten genes (. ugp, slc35a2, ganc, man1a, man1c, mgat5a, st3gal5, glb1, neu1, and neu3) were up-regulated and three genes (. b4galt2, st3gal3, and neu2) were down-regulated significantly. Altered expression patterns in st3gal3, neu1, and neu3, which have roles in the sialic acid biosynthesis pathway, correlated with reduced sialic acid content of the glycoprotein by NaBu. Taken together, the results obtained in this study provide a better understanding of the detrimental effect of NaBu on N-glycosylation in rCHO cells.

ISSN 

0168-1656

Link 

http://dx.doi.org/10.1016/j.jbiotec.2013.12.001

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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