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Title 

Whole genome resequencing of Heugu (Korean black cattle) for the genome-wide SNP discovery

Authors 

J W ChoiWon Hyong JungK T LeeK S JungY ChoNamshin KimT H Kim

Issue Date 

2013

Citation 

Korean Journal for Food Science of Animal Resources, vol. 33, no. 6, pp. 715-722

Keywords 

HeuguKorean Black CattleSingle nucleotide polymorphismsWhole genome resequencing

Abstract 

Heugu (Korea Black Cattle) is one of the indigenous cattle breeds in Korea; however there has been severe lack of genomic studies on the breed. In this study, we report the first whole genome resequencing of Heugu at higher sequence coverage using Illumina HiSeq 2000 platform. More than 153.6 Giga base pairs sequence was obtained, of which 97% of the reads were mapped to the bovine reference sequence assembly (UMD 3.1). The number of non-redundantly mapped sequence reads corresponds to approximately 28.9-fold coverage across the genome. From these data, we identified a total of over six million single nucleotide polymorphisms (SNPs), of which 29.4% were found to be novel using the single nucleotide polymorphism database build 137. Extensive annotation was performed on all the detected SNPs, showing that most of SNPs were located in intergenic regions (70.7%), which is well corresponded with previous studies. Of the total SNPs, we identified substantial numbers of non-synonymous SNPs (13,979) in 5,999 genes, which could potentially affect meat quality traits in cattle. These results provide genome-wide SNPs that can serve as useful genetic tools and as candidates in searches for phenotype-altering DNA difference implicated with meat quality traits in cattle. The importance of this study can be further pronounced with the first whole genome sequencing of the valuable local genetic resource to be used in further genomic comparison studies with diverse cattle breeds.

ISSN 

1225-8563

Link 

http://dx.doi.org/10.5851/kosfa.2013.33.6.715

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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