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Title 

ER stress-inducible ATF3 suppresses BMP2-induced ALP expression and activation in MC3T3-E1 cells

Authors 

Jae Kyung ParkHoon JangS HwangE J KimDong Ern KimK B OhD J KwonJ T KohK KimuraH InoueW G JangJeong Woong Lee

Publisher 

Elsevier

Issue Date 

2014

Citation 

Biochemical and Biophysical Research Communications, vol. 443, no. 0, pp. 333-338

Keywords 

Activating transcription factor 3 (ATF3)

Abstract 

Endoplasmic reticulum (ER) stress suppresses osteoblast differentiation. Activating transcription factor (ATF) 3, a member of the ATF/cAMP response element-binding protein family of transcription factors, is induced by various stimuli including cytokines, hormones, DNA damage, and ER stress. However, the role of ATF3 in osteoblast differentiation has not been elucidated. Treatment with tunicamycin (TM), an ER stress inducer, increased ATF3 expression in the preosteoblast cell line, MC3T3-E1. Overexpression of ATF3 inhibited bone morphogenetic protein 2-stimulated expression and activation of alkaline phosphatase (ALP), an osteogenic marker. In addition, suppression of ALP expression by TM treatment was rescued by silencing of ATF3 using shRNA. Taken together, these data indicate that ATF3 is a novel negative regulator of osteoblast differentiation by specifically suppressing ALP gene expression in preosteoblasts.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2013.11.121

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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