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Title 

SIPMEI, a pollen-specific gene in tomato

Authors 

Woong Bom KimC J LimHyun A JangSo Young YiS K OhH Y LeeHyun A KimY I ParkSuk Yoon Kwon

Publisher 

Agricultural Institute of Canada

Issue Date 

2014

Citation 

Canadian Journal of Plant Science, vol. 94, no. 1, pp. 73-83

Keywords 

ArabidopsisPMEIPollen-specific geneSlPMEI promoterStressTomato

Abstract 

Pectin is one of the main components of plant cell walls, and its biosynthesis is controlled by pectin methylesterase (PME). Pectin methylesterase inhibitors (PMEIs) are key regulators of PME. We report here the cloning and characterization of a novel Solanum lycopersicum L. PMEI gene, SlPMEI. RT-PCR studies of leaf, seed, fruit, flower, and flower organs confirmed that SlPMEI is expressed specifically in pollen. Promoter analysis of SlPMEI revealed pollen-specific cis-acting elements (pollen lat52 and g10). In addition, SlPMEI is expressed independently of abiotic stress, pathogen exposure, and growth stage in tomato, and a histochemical analysis of promoter activity revealed pollen-specific expression in both Arabidopsis and tomato. Under the microscope, we observed pollen-specific GUS expression in the stamen of transgenic tomato plant. These results indicate that the promoter of SlPMEI has strong pollen-specific activity, and could therefore be useful for development of industrially and agronomically important transgenic plants

ISSN 

0008-4220

Link 

http://dx.doi.org/10.4141/CJPS2013-084

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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