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Title 

Production of isobutanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain

Authors 

Baek Rock OhSun-Yeon HeoS M LeeWon-Kyung HongJang Min ParkYou Ree JungD H KimJung Hoon SohnJeong-Woo SeoChul Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2014

Citation 

Biotechnology Letters, vol. 36, no. 1, pp. 57-62

Keywords 

2-Butanol/isobutanolCrude glycerolGenetic engineeringKlebsiella pneumoniae

Abstract 

Klebsiella pneumoniae was engineered to produce 2-butanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, {increment}ldhA/pBR-iBO (ilvIH-ilvC-ilvD-kivd-adhA), produced 2-butanol (160 mg l-1) from crude glycerol. To increase the yield of 2-butanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of 2-butanol from 160 to 320 mg l-1. This represents the first successful attempt to produce 2-butanol from crude glycerol.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-013-1333-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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