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Title 

Identification of the novel substrates for caspase-6 in apoptosis using proteomic approaches

Authors 

Jin Hwa ChoPhil Young LeeW C SonSeung-Wook ChiByoung Chul ParkJeong Hoon KimSung Goo Park

Publisher 

Korean Society for Biochemistry and Molecular Biology

Issue Date 

2013

Citation 

BMB Reports, vol. 46, no. 12, pp. 588-593

Keywords 

ApoptosisCaspase-6DegradomicsProteomic screeningSubstrate

Abstract 

Apoptosis, programmed cell death, is a process involved in the development and maintenance of cell homeostasis in multicellular organisms. It is typically accompanied by the activation of a class of cysteine proteases called caspases. Apoptotic caspases are classified into the initiator caspases and the executioner caspases, according to the stage of their action in apoptotic processes. Although caspase-3, a typical executioner caspase, has been studied for its mechanism and substrates, little is known of caspase-6, one of the executioner caspases. To understand the biological functions of caspase-6, we performed proteomics analyses, to seek for novel caspase-6 substrates, using recombinant caspase-6 and HepG2 extract. Consequently, 34 different candidate proteins were identified, through 2-dimensional electrophoresis/MALDI-TOF analyses. Of these identified proteins, 8 proteins were validated with in vitro and in vivo cleavage assay. Herein, we report that HAUSP, Kinesin5B, GEP100, SDCCAG3 and PARD3 are novel substrates for caspase-6 during apoptosis.

ISSN 

1976-6696

Link 

http://dx.doi.org/10.5483/BMBRep.2013.46.12.081

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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