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Title | Optimized production of 2,3-butanediol by a lactate dehydrogenase-deficient mutant of Klebsiella pneumoniae |
| Lactate dehydrogenase 결손 Klebsiella pneumoniae를 사용한 2,3-Butanediol 최적 생산 |
Authors | S B Lee; Baek Rock Oh; Jang Min Park; A Yu; Sun-Yeon Heo; Won-Kyung Hong; Jeong-Woo Seo; Chul Ho Kim |
Publisher | Springer Verlag (Germany) |
Issue Date | 2013 |
Citation | Biotechnology and Bioprocess Engineering, vol. 18, no. 0, pp. 1210-1215 |
Keywords | 2,3-butanediol; Klebsiella pneumoniae; lactate dehydrogenasedeficient mutant; response surface methodology; sugarcane molasses |
Abstract | To obtain high-yield production of 2,3-butanediol (2,3-BD) from glucose, we optimized the culture conditions for a lactate dehydrogenase-deficient mutant (ΔldhA) of Klebsiella pneumoniae using response surface methodology. 2,3-BD production was successfully improved by optimizing pH (5.6), aeration (3.50 vvm) and concentration of corn steep liquor (45.0 mL/L) as a nitrogen source, resulting in a maximum level of 2,3-BD production of 148.8 g/L and productivity of 2.48 g/L/h. 2,3-BD was also obtained with high concentration (76.24 g/L) and productivity (2.31 g/L/h) from the K. pneumoniae mutant strain using sugarcane molasses as a carbon source. |
ISSN | 1226-8372 |
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Registered Date |
2019-05-02 |
