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Title 

Glaciihabitans tibetensis gen. nov., sp. nov., a psychrotolerant bacterium of the family Microbacteriaceae, isolated from glacier ice water

Authors 

A H LiH C LiuY H XinSong-Gun KimY G Zhou

Publisher 

Society for General Microbiology

Issue Date 

2014

Citation 

International Journal of Systematic and Evolutionary Microbiology, vol. 64, no. 2, pp. 579-587

Abstract 

A Gram-stain-positive, aerobic, non-spore-forming, short-rod-shaped bacterium, designated strain MP203T, was isolated from ice water of Midui Glacier in Tibet Autonomous Region, China. The strain was psychrotolerant, growing at 0-25 °C. 16S rRNA gene sequence analysis showed that strain MP203T was most similar to Frigoribacterium faeni NBRC 103066T, Compostimonas suwonensis KACC 13354T, Frigoribacterium mesophilum KCTC 19311T, Marisediminicola antarctica CCTCC AB 209077T and Alpinimonas psychrophila JCM 18951T, with similarities of 97.4, 97.2, 97.2, 97.1 and 97.1 %, respectively. The maximum-likelihood phylogenetic tree indicated that strain MP203T clustered with nine genera of the family Microbacteriaceae, namely Frigoribacterium, Compostimonas, Marisediminicola, Alpinimonas, Frondihabitans, Clavibacter, Subtercola, Klugiella and Agreia. However, bootstrap analysis showed that there was no significance in the branching pattern of the linage comprising strain MP203T and any existing generic lineage of the family Microbacteriaceae. DNA-DNA hybridization results indicated levels of relatedness between strain MP203T and Marisediminicola antarctica CCTCC AB 209077T, Frigoribacterium faeni NBRC 103066T, Frigoribacterium mesophilum KCTC 19311T, Compostimonas suwonensis KACC 13354T and Alpinimonas psychrophila JCM 18951T were 25.8±7.3, 29.6±7.6, 19.7±6.7, 16.0±4.2 and 12.4±5.1 % (mean±SD), respectively. The G+C content of the genomic DNA was 64.1 mol%. Analysis of the cell-wall peptidoglycan revealed that the peptidoglycan structure of strain MP203T was B10 type with Gly[L-Hse]-D-Glu-D-DAB, containing 2, 4-diaminobutyric acid (DAB) as a diagnostic amino acid. The cell-wall sugars were rhamnose, ribose, mannose and glucose. The major fatty acids were anteiso-C15: 0, iso-C16: 0 and anteiso A-C15: 1. An unusual compound identified as anteiso-C15: 0-DMA (1, 1-dimethoxy-anteiso-pentadecane) was also present in strain MP203T. The predominant menaquinone was MK-10. Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one unknown glycolipid and four unknown lipids were detected in the polar lipid extracts. As strain MP203T was distinguishable from phylogenetically related genera in the family Microbacteriaceae in terms of its physiological and chemotaxonomic characteristics and phylogenetic position, it was considered to represent a novel species of a new genus. Thus, the name Glaciihabitans tibetensis gen. nov., sp. nov. is proposed. The type strain of Glaciihabitans tibetensis is MP203T (= CGMCC 1.12484T = KCTC 29148T)

ISSN 

1466-5026

Link 

http://dx.doi.org/10.1099/ijs.0.052670-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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