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Title 

Fourier transform infrared (FT-IR) spectroscopy of genomic DNA to discriminate F1 progenies from their paternal lineage of Chinese cabbage (Brassica rapa subsp. pekinensis)

Authors 

S Y SongEun-Yee JieMyung-Suk AhnI H LeeI S NouB W MinSuk Weon Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2014

Citation 

Molecular Breeding, vol. 33, no. 2, pp. 453-464

Keywords 

Chinese cabbageDiscriminationFT-IRGenomic DNAMultivariate analysisProgeny

Abstract 

Fourier transform infrared spectroscopy (FT-IR) spectroscopy in combination with multivariate analysis was used to discriminate two different F1 hybrid lines from their parental inbred lines. Genomic DNA was isolated from leaves of three parental lines and two F1 hybrid lines of Brassica campestris subsp. pekinensis. Purified genomic DNA was analyzed by FT-IR spectroscopy in the spectral region from 4,000 to 400 cm-1. FT-IR spectra confirmed typical spectral differences between the frequency regions of N-H stretching (amide I) and C=O stretching vibrations (amide II) as well as PO2 - ionized asymmetric and symmetric stretching. Principal component analysis was able to discriminate between F1 hybrid progenies depending on their parental lineages, even though they share the same maternal background. Partial least squares discriminant analysis gave a more clear discrimination between the two parental lines and their hybrid progenies. These FT-IR spectral differences might be directly related to subtle changes in the base functional group and backbone structures of genomic DNA. Considering these results, this technique could provide a solid research foundation for FT-IR spectral-based rapid diagnosis, selection, and discrimination of parental lines from their progenies. Furthermore, this technique could be applied to test purity in the hybrid seed industry.

ISSN 

1380-3743

Link 

http://dx.doi.org/10.1007/s11032-013-9963-4

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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