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Title 

Bioengineered bacterial outer membrane vesicles as cell-specific drug-delivery vehicles for cancer therapy

Authors 

V GujratiS KimSang-Hyun KimJ J MinH E ChoyS C KimS Jon

Publisher 

American Chemical Society

Issue Date 

2014

Citation 

ACS Nano, vol. 8, no. 2, pp. 1525-1537

Keywords 

affibodycancer therapydrug deliveryouter membrane vesiclessiRNA

Abstract 

Advances in genetic engineering tools have contributed to the development of strategies for utilizing biologically derived vesicles as nanomedicines for achieving cell-specific drug delivery. Here, we describe bioengineered bacterial outer membrane vesicles (OMVs) with low immunogenicity that can target and kill cancer cells in a cell-specific manner by delivering small interfering RNA (siRNA) targeting kinesin spindle protein (KSP). A mutant Escherichia coli strain that exhibits reduced endotoxicity toward human cells was engineered to generate OMVs displaying a human epidermal growth factor receptor 2 (HER2)-specific affibody in the membrane as a targeting ligand. Systemic injection of siRNA-packaged OMVs caused targeted gene silencing and induced highly significant tumor growth regression in an animal model. Importantly, the modified OMVs were well tolerated and showed no evidence of nonspecific side effects. We propose that bioengineered OMVs have great potential as cell-specific drug-delivery vehicles for treating various cancers.

ISSN 

1936-0851

Link 

http://dx.doi.org/10.1021/nn405724x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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