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Title 

Inhibition effect of flavonoid compounds against neuraminidase expressed in Pichia pastoris

 

Flavonoid 화합물들의 뉴라미니데이즈 저해효과

Authors 

T T H NguyenH K KangYoung-Min KimD Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2014

Citation 

Biotechnology and Bioprocess Engineering, vol. 19, no. 0, pp. 70-75

Keywords 

catechinflavonoidH5N1molecular dockingneuraminidasePichia pastoris

Abstract 

Neuraminidase (NA) is one of the two glycoproteins on the surface of influenza virus, which cleaves terminal sialic acid residues and facilitates the release of virions from infected cells. The recombinant NA from H5N1 influenza virus strain A/Vietnam/1203/04 was expressed in Pichia pastoris X33 as a 45 kDa protein that displayed a K m of 9.96 ± 1.26 μM with fluorogenic substrate, 2′-(4-methylumbelliferyl)-α-D-N-acetyl neuraminic acid. Partially purified NA was used for the inhibition and kinetic assays with eight flavonoid compounds and gallic acid. Among them, gallocatechin gallate (GCG) showed the best inhibition against NA with the IC50 of 8.98 ± 0.46 μM and showed a competitive inhibition pattern with K i value of 8.34 ± 0.25 μM. In molecular docking experiments, GCG displayed a binding energy of -13.71 kcal/mol to the active site of NA and the galloyl moiety was required for NA inhibition activity.

ISSN 

1226-8372

Link 

http://dx.doi.org/10.1007/s12257-013-0599-3

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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