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Title 

Valproic acid enhances early development of bovine somatic cell nuclear transfer embryos by alleviating endoplasmic reticulum stress

 

VPA 처리가 체세포 복제수정란의 초기배 발달에 미치는 영향

Authors 

Bong Seok SongSeung-Bin YoonBo Woong SimKim Young-HyunCha Jae JinSeon-A ChoiKang Jin JeongKim Ji-SuJae Won HuhSang-Rae LeeKim Sang-HyunSun-Uk KimKyu Tae Chang

Publisher 

CSIRO Publishing

Issue Date 

2014

Citation 

Reproduction Fertility and Development, vol. 26, no. 3, pp. 432-440

Keywords 

ApoptosisBlastocystDevelopmental competenceHistone acetylationReprogramming

Abstract 

Despite the positive roles of histone deacetylase inhibitors in somatic cell nuclear transfer (SCNT), few studies have evaluated valproic acid (VPA) and its associated developmental events. Thus, the present study was conducted to elucidate the effect of VPA on the early development of bovine SCNT embryos and the underlying mechanisms of action. The histone acetylation level of SCNT embryos was successfully restored by VPA, with optimal results obtained by treatment with 3mM VPA for 24h. Importantly, the increases in blastocyst formation rate and inner cell mass and trophectoderm cell numbers were not different between the VPA and trichostatin A treatment groups, whereas cell survival was notably improved by VPA, indicating the improvement of developmental competence of SCNT embryos by VPA. Interestingly, VPA markedly reduced the transcript levels of endoplasmic reticulum (ER) stress markers, including sXBP-1 and CHOP. In contrast, the levels of GRP78/BiP, an ER stress-alleviating transcript, were significantly increased by VPA. Furthermore, VPA greatly reduced cell apoptosis in SCNT blastocysts, which was further evidenced by the increased levels of the anti-apoptotic transcript Bcl-xL and decreased level of the pro-apoptotic transcript Bax. Collectively, these results suggest that VPA enhances the developmental competence of bovine SCNT embryos by alleviating ER stress and its associated developmental damage.

ISSN 

1031-3613

Link 

http://dx.doi.org/10.1071/RD12336

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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