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Title 

Highly stable colorimetric aptamer sensors for detection of ochratoxin A through optimizing the sequence with the covalent conjugation of hemin

Authors 

Jayeon LeeChang Hoon JeonS J AhnTai Hwan Ha

Publisher 

Royal Society of Chemistry

Issue Date 

2014

Citation 

Analyst, vol. 139, no. 7, pp. 1622-1627

Abstract 

Optimization of hairpin DNA is introduced to detect ochratoxin A (OTA) by chemically conjugating its cofactor, hemin, toward the 5′-end. The newly designed OTA aptasensor showed enhanced stability and sensitivity, thereby lowering the detection limit to an ∼1 nM level. Furthermore, an optimal spacer for hemin conjugation was investigated for stable responses toward very diluted OTA solutions.

ISSN 

0003-2654

Link 

http://dx.doi.org/10.1039/c3an01639k

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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