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Title 

A regeneratable, label-free, localized surface plasmon resonance (LSPR) aptasensor for the detection of ochratoxin A

Authors 

J H ParkJ Y ByunH MunW B ShimYong Beom ShinT LiM G Kim

Publisher 

Elsevier

Issue Date 

2014

Citation 

Biosensors & Bioelectronics, vol. 59, no. C, pp. 321-327

Keywords 

AptamerGold nanorodLabel-free detectionLocalized surface plasmon resonanceOchratoxin ASmall molecule

Abstract 

Binding of an analyte on the surface of a nanoparticle typically promotes a change in the local refractive index, which gives rise to a shift in the wavelength of the localized surface plasmon resonance (LSPR) absorption band. The magnitude of the LSPR wavelength change is dependent on both the location of the analyte relative to the surface of the nanoparticle and the degree of alteration of the refractive index. We have employed this phenomenon as the basis for designing a new, label-free approach for the detection of the toxic mold mycotoxin, ochratoxin A (OTA) that employs a gold nanorod (GNR) and an aptamer target binding mechanism. In this system, binding of OTA causes an accumulation of OTA and G-quadruplex structure of the aptamer. This process results in a longitudinal wavelength shift of the LSPR peak associated with a change in the local refractive index near the GNR surface. By using this method, OTA can be quantitatively detected at concentrations lower than 1. nM. In addition, the results of this effort show that aptamer functionalized GNR substrate is robust in that it can be regenerated for reuse over seven times by heating in methanol at 70. °C to remove OTA. Moreover, the proposed biosensor system exhibits high selectivity for OTA over other mycotoxins. Finally, the sensor can be employed to detect OTA in ground corn samples with excellent recovery levels.

ISSN 

0956-5663

Link 

http://dx.doi.org/10.1016/j.bios.2014.03.059

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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