상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Donor-derived natural killer cells infused after human leukocyte antigen-haploidentical hematopoietic cell transplantation: a dose-escalation study

Authors 

In Pyo ChoiSuk Ran YoonSoo Yeon ParkHanna KimSol-Ji JungYe Jin JangMin Ho KangYoung Il YeomJ L LeeD Y KimY S LeeY A KangM JeonM SeolJ H LeeH J KimS C YunK H Lee

Publisher 

Elsevier

Issue Date 

2014

Citation 

Biology of Blood and Marrow Transplantation, vol. 20, no. 5, pp. 696-704

Keywords 

Donor natural killer cell infusionHuman leukocyte antigen-haploidentical hematopoietic cell transplantation

Abstract 

The doses of donor-derived natural killer (NK) cells that can be given safely after human leukocyte antigen (HLA)-haploidentical hematopoietic cell transplantation (HCT) remain to be defined. Forty-one patients (ages 17 to 75years) with hematologic malignancy underwent HLA-haploidentical HCT after reduced-intensity conditioning containing busulfan, fludarabine, and antithymocyte globulin. Cell donors (ages 7 to 62years) underwent growth factor-mobilized leukapheresis for 3 to 4days. Cells collected on the first 2 to 3days were used for HCT, whereas those collected on the last day were CD3-depleted and cultured into NK cells using human interleukins-15 and -21. These NK cells were then infused into patients twice at 2 and 3weeks after HCT at an escalating doses of.2×108cells/kg of body weight (3 patients), 5×108cells/kg (3 patients), 1.0×108cells/kg (8 patients), and ≥ 1.0×108cells/kg or available cells (27 patients). At all dose levels, no acute toxicity was observed after NK cell infusion. After HLA-haploidentical HCT and subsequent donor NK cell infusion, when referenced to 31 historical patients who had undergone HLA-haploidentical HCT after the same conditioning regimen but without high-dose NK cell infusion, there was no significant difference in the cumulative incidences of major HCT outcomes, including engraftment (absolute neutrophil count≥500/μL, 85% versus 87%), grade 2 to 4 acute graft-versus-host disease (GVHD, 17% versus 16%), moderate to severe chronic GVHD (15% versus 10%), and transplantation-related mortality (27% versus 19%). There was, however, a significant reduction in leukemia progression (74% to 46%), with post-transplantation NK cell infusion being an independent predictor for less leukemia progression (hazard ratio, 527). Our findings showed that, when given 2 to 3weeks after HLA-haploidentical HCT, donor-derived NK cells were well tolerated at a median total dose of 2.0×108cells/kg. In addition, they may decrease post-transplantation progression of acute leukemia.

ISSN 

1083-8791

Link 

http://dx.doi.org/10.1016/j.bbmt.2014.01.031

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)