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Title 

Variable allelic expression of imprinted genes in human pluripotent stem cells during differentiation into specialized cell types in vitro

Authors 

S W ParkJ KimJong Lyul ParkJ Y KoI ImH S DoH KimN T TranS H LeeYong Sung KimYee Sook ChoD R LeeY M Han

Publisher 

Elsevier

Issue Date 

2014

Citation 

Biochemical and Biophysical Research Communications, vol. 446, no. 2, pp. 493-498

Keywords 

Allele-specific expressionHuman pluripotent stem cellsImprinted genesIn vitro differentiation

Abstract 

Genomic imprinting is an epigenetic phenomenon by which a subset of genes is asymmetrically expressed in a parent-of-origin manner. However, little is known regarding the epigenetic behaviors of imprinted genes during human development. Here, we show dynamic epigenetic changes in imprinted genes in hESCs during in vitro differentiation into specialized cell types. Out of 9 imprinted genes with single nucleotide polymorphisms, mono-allelic expression for three imprinted genes (H19, KCNQ1OT1, and IPW), and bi- or partial-allelic expression for three imprinted genes (OSBPL5, PPP1R9A, and RTL1) were stably retained in H9-hESCs throughout differentiation, representing imprinting stability. Three imprinted genes (KCNK9, ATP10A, and SLC22A3) showed a loss and a gain of imprinting in a lineage-specific manner during differentiation. Changes in allelic expression of imprinted genes were observed in another hESC line during in vitro differentiation. These findings indicate that the allelic expression of imprinted genes may be vulnerable in a lineage-specific manner in human pluripotent stem cells during differentiation.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2014.02.141

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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