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Title 

Development of the large-scale oligonucleotide chip for the diagnosis of plant viruses and its practical use

Authors 

M NamJ S KimSeungmo LimC Y ParkJ G KimH S ChoiH S LimJae Sun MoonS H Lee

Publisher 

Han Rim Won Publishing Company

Issue Date 

2014

Citation 

Plant Pathology Journal, vol. 30, no. 1, pp. 51-57

Keywords 

Large-scale oligonucleotide chipLSON chipOligo chipPlant virusVirus diagnosis

Abstract 

A large-scale oligonucleotide (LSON) chip was developed for the detection of the plant viruses with known genetic information. The LSON chip contains two sets of 3,978 probes for 538 species of targets including plant viruses, satellite RNAs and viroids. A hundred forty thousand probes, consisting of isolate-, species- and genus-specific probes respectively, are designed from 20,000 of independent nucleotide sequence of plant viruses. Based on the economic importance, the amount of genome information, and the number of strains and/or isolates, one to fifty-one probes for each target virus are selected and spotted on the chip. The standard and field samples for the analysis of the LSON chip have been prepared and tested by RT-PCR. The probe's specific and/or nonspecific reaction patterns by LSON chip allow us to diagnose the unidentified viruses. Thus, the LSON chip in this study could be highly useful for the detection of unexpected plant viruses, the monitoring of emerging viruses and the fluctuation of the population of major viruses in each plant.

ISSN 

1598-2254

Link 

http://dx.doi.org/10.5423/PPJ.OA.08.2013.0084

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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